The committed differentiation of bone marrow mesenchymal stem cells into tenocytes
- VernacularTitle:BMP12诱导恒河猴骨髓间充质干细胞定向分化为腱细胞
- Author:
Qiwei WANG
;
Zhaolie CHEN
;
Yingjie PIAO
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
bone morphogenetic protein 12;
committed differentiation;
gene transfection;
tenocytes
- From:
Medical Journal of Chinese People's Liberation Army
2005;30(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of committed differentiation of rhesus bone marrow mesenchymal stem cells (MSCs) into tenocytes induced by BMP12. Methods MSCs were transfected with pTARGETTM bearing BMP12 gene by electroporation. The transfected cells were identified by morphological observation and molecular biological measure. Results Under the observation of light microscope, the morphological features of transfected cells changed significantly compared to parental MSCs. RT-PCR data showed the transfected cells had the mRNA expression of BMP12 and collagen Ⅰ, but without that of collagen Ⅲ. 98.39% of the transfected cells were CD44+ and negative for HLA-DR. Conclusion BMP12 could induce MSCs into tenocytes, and bone marrow MSCs might be the optional seed cells for tendon tissue engineering.
