Regulative Effects of Hawthorn Leave Flavonoids on Cytotoxicity,NO and Ca2+in Hypoxia-treated Human Umbilical Vein Endothelial Cells
- VernacularTitle:山楂叶总黄酮对缺氧人脐静脉内皮细胞细胞毒、NO和Ca2+水平的调控作用
- Author:
Wenjun LAN
;
Yakun GE
;
Xiaoxiang ZHENG
- Publication Type:Journal Article
- Keywords:
hawthorn leave flavonoids;
umbilical vein;
endothelial cells;
cytotoxicity;
NO;
Ca2+
- From:Space Medicine & Medical Engineering
2005;18(3):157-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the potential effect of HLF (Hawthorn leave flavonoids, w/w, 80% flavonoids) against thrombus formation, effect of HLF on hypoxia-treated human umbilical vein endothelial cell (HUVECs) was studied. Method The levels of cytotoxicity and NO upon HUVECs were studied by flow cytometry. Moreover, the level of calcium ion in HUVECs was examined through laser scanning confocal microscopy. Result Data from this study showed that HLF at concentrations of 5 μg/ml and 10 μg/ml decreased the cytotoxicity of hypoxia to HUVECs (P<0.05, P<0.01). The intracellular levels of NO and calcium ion were downregulated by HLF at concentrations of 5 μg/ml (P<0.01; P<0.01) and 10 μg/ml (vs control, P<0.01; P<0.01) too. Conclusion Results observed suggest that HLF protect HUVECs from hypoxia partly through its regulative effect on NO and calcium ion levels.
