Preparation and blood sugar lowering effect of oral chitosan-insulin nanoparticles on diabetic rats
- VernacularTitle:口服壳聚糖胰岛素纳米粒的制备及其降血糖作用研究
- Author:
Baojun DONG
;
Changyong WANG
;
Ximin GUO
;
Rongqi WANG
;
Haixia MA
;
Lingzhi DONG
;
Ming FAN
- Publication Type:Journal Article
- Keywords:
chitosan;
insulin;
nanoparticles;
hypoglycemic effect
- From:
Medical Journal of Chinese People's Liberation Army
2005;30(3):208-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the method of preparation and blood sugar lowering effect of oral chitosan-insulin nanoparticles (INS-NPs) in streptozotocin-induced diabetic Wistar rats. Methods The INS-NPs were prepared by an ionic gelation method. The changes in the morphology and size of the INS-NPs were observed with transmission electron microscope and Zetasizer 3000HS, respectively. The blood sugar lowering effect of the INS-NPs was evaluated by monitoring the blood glucose levels in healthy and streptozotocin-induced diabetic rats. Results INS-NPs were spherical in shape with a mean size of 220.6±15.9nm. Entrapment efficiency of INS-NPs was 75.4%±3.2% and the loading efficiency of INS-NPs was 19.5%±2.6%. In vivo blood sugar lowering study showed that the levels of blood glucose of healthy Wistar rats were significantly reduced from 6h to 12h after oral administration of INS-NPs(25U/kg). The blood glucose level of diabetic rats were significantly reduced at 6h after oral administration of INS-NPs (25U/kg), and this effect was maintained for more than 9h, and the levels of blood glucose were kept in normal range for 7h. Conclusion The INS-NPs prepared by ionic gelation method has the blood glucose lowering effect in streptozotocin-induced diabetic Wistar rats.
