Effects of Gingko biloba extract on glutamate-induced [ Ca2+ ]i changes in cultured cortical astrocytes after hypoxia/reoxygenation, H2O2 or L-glutamate injury
- VernacularTitle:银杏叶提取物对低氧复氧、H2O2和谷氨酸损伤时谷氨酸引起的大鼠星形胶质细胞[Ca2+]i变化的影响
- Author:
Zhen LI
;
Xianming LIN
;
Peili GONG
;
Guanhua DU
;
Fandian ZENG
- Publication Type:Journal Article
- Keywords:
Gingko biloba extract;
astrocyte;
[ Ca2+ ]i;
hypoxia/reoxygenation;
glutamate;
H2O2
- From:
Acta Pharmaceutica Sinica
2005;40(3):213-219
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate glutamate-induced [ Ca2 + ] i changes in cultured rat neonatal cortical astrocytes after hypoxia/reoxygenation, H2O2 or high concentration of L-glutamate injury. In the meantime, the effects of Gingko biloba extract (GbE) were examined. Methods [ Ca2+ ]i changes in astrocytes were monitored by laser scanning confocal microscopy with the Ca2+ sensitive fluorescent probe [ Ca2 + ] i, but decrease by ( 3.3 ± 1.6) %, (81 ± 11 ) % and ( 81 ± 7 ) %, respectively. Pretreatment with H2O2 or high concentration of L-glutamate injury were ( 135 ± 98 ) %, ( 117 ± 93 ) % and ( 89 ± 36) %,different injury. Conclusion Hypoxia/reoxygenation, H2O2 and high concentration of L-glutamate impaired astrocytes' response to exogenous L-glutamate, and then bidirectional communication between astrocytes and neurons could not take place. GbE could improve the abnormal responses and maintain the normal function of astroglical network. These effects support that GbE has potential beneficial actions against brain injury.
