Protective effects of hydroxyethylpuerarin on cultured bovine cerebral microvascular endothelial cells damaged by hydrogen peroxide
- VernacularTitle:羟乙基葛根素对过氧化氢致牛脑微血管内皮细胞损伤的保护作用
- Author:
Hongmei GUANG
;
Xiumei ZHANG
;
Yingquan LI
;
Xinbing WEI
;
Ziying WANG
;
Huiqing LIU
- Publication Type:Journal Article
- Keywords:
hydroxyethylpuerarin;
bovine cerebral microvascular endothelial cells;
vascular endothelium;
apoptosis;
antioxidants
- From:
Acta Pharmaceutica Sinica
2005;40(3):220-224
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the damages induced by hydrogen peroxide in cultured bovine cerebral microvascular endothelial cells (BCMEC) and evaluate the protective effects of hydroxyethylpuerarin on hydrogen peroxide-injured BCMEC. Methods BCMEC were cultured and transferred into modified Eagle medium (MEM). The viability of cells was detected by MTT assay. Cell injury was determined by lactate dehydrogenase (LDH) activity in the extracellular medium. Flow cytometry was employed to observe the occurrence of apoptosis. Morphologic changes of cells were visualized under phase contrast and electron occurrence of apoptosis. Hydroxyethylpuerarin was shown to increase the survival rate and decrease the activity of LDH of BCMEC damaged by hydrogen peroxide. Hydroxyethylpuerarin was also found to protect BCMEC against apoptosis induced by hydrogen peroxide. Conclusion Hydrogen peroxide induces BCMEC injury either by apoptosis or through necrosis. Hydroxyethylpuerarin protects BCMEC against hydrogen peroxide-induced injury in a concentration-dependent manner. Its antioxidant effects might be involved as the mechanism protection.
