Determination of Circulating Antigen in Neurocysticercosis Patients by Monoclonal Antibody
- VernacularTitle:应用单克隆抗体检测脑囊虫病患者的循环抗原
- Author:
Zengzhu GUO
;
Minjun HUANG
;
Yijun AN
;
Song HUANG
;
Hongjie JIANG
- Publication Type:Journal Article
- Keywords:
Cysticercus cellulosae;
neurocysticercosis;
circulating antigen (CAg);
monoclonal antibody;
ELISA
- From:
Journal of Tropical Medicine
2005;5(2):113-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect circulating antigen (Cag) for diagnosing neurocysticercosis. Method ELISA was performed with monoclonal antibody 4B6 against the cyst fluid antigen of Cyticercus cellulosae for detecting Cag in serum and/or cerebrospinal fluid (CSF) of patients with neurocysticercosis or with other diseases. Results In the group of 82 cases of neurocysticercosis, the positive rate of serum Cag was 79.2% (65/82) and the positive rate of CSF Cag was 100% (26/26). After chemotherapy for 20 cases with positive serum Cag, the titer of serum Cag in 17 cases dropped to zero(85% ). Cag could not be detected in specimens from patients with other diseases. Conclusion These results indicate that the determination of Cag, especially of the CSF Cag, is useful for the diagnosis of neurocysticercosis and the drop in serum Cag is a good parameter for the evaluation of the effectiveness of chemotherapy.
