A LIF Mutation at The 29th Amino Acid Totally Abolished The Biological Functions
- VernacularTitle:白血病抑制因子LIF第29位氨基酸突变导致其功能完全丧失
- Author:
Hongxiu NING
;
Yue CHEN
;
Yuanjiang ZHANG
;
Yu RONG
;
Xiaojun WU
;
Xiufang ZHANG
;
Zhijie CHANG
- Publication Type:Journal Article
- Keywords:
leukemia inhibitory factor(LIF);
mutation;
functions;
proliferation
- From:
Progress in Biochemistry and Biophysics
2005;32(4):318-324
- CountryChina
- Language:Chinese
-
Abstract:
Leukemia inhibitory factor (LIF) plays important roles in varieties of biological processes. This factor is highly conserved in mammalian animals and only one heterozygous LIF mutation was reported to cause the infertility of women. A LIF mutation was generated and the evidences were provided that the mutation of mature LIF at the 29th amino acid totally abolished its functions, including stimulation of STAT activation assayed by Luciferase reporter gene expression and EMSA experiments. In addition, the mutated LIF failed to inhibit the proliferation of M1 cells. The data indicated that the mutation of LIF did not have a dominant negative effect but lost the biological functions, suggesting that the 29th amino acid is critical for maintaining the activities of LIF.
