HIGH CONCENTRATION OF IRON IN CEREBROSPINAL FLUID OF RATS INDUCES ALZHEIMER-LIKE BEHAVIORAL AND PATHOLOGICAL CHANGES
- VernacularTitle:大鼠脑脊液中高浓度铁诱导的Alzheimer病样行为学和病理学变化
- Author:
Lin LI
;
Jianliang WU
;
Zheng JIN
;
Yan DOU
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
Iron;
Apoptosis;
Morris water maze;
β amyloid protein
- From:
Chinese Journal of Neuroanatomy
2005;21(3):252-258
- CountryChina
- Language:Chinese
-
Abstract:
In order to estimate the relationship between iron and the Alzheimer's disease, the behavioral and pathological changes were observed by Morris water maze and immunohistochemical staining respectively after injecting FeC13 into brain ventricle of rats. The apoptosis was tested by flow cytometry and the electron microscopy was used to observe ultrastructural changes. There were significant differences in escape latency of time and distance between normal animals and iron treated rats. Percentage and fluorescence intension (FI) of AnnexinV FITC loaded cells undergoing apoptosis were higher in iron treated rats compared with normal animals. Fawn-coloured products of β amyloid protein were interspersedly distributed in extensive areas of cerebral cortex and hippocampus. Under electron microscope, vacuolate degeneration of neuronal processes with mitochondria degeneration and accumulation of microtubule near vacuolar nucleus were observed in iron treated rats. These results suggest that a local higher concentration of iron in brain may induce Alzheimer-like impairment of intelligence and pathological changes.
