In vitro study of atrial natriuretic peptide circadian secreted by encapsulated atrial natriuretic peptide cDNA transfection cells
- VernacularTitle:微囊化转心房肽基因细胞分泌心房肽近日节律的体外研究
- Author:
Yonghang FU
;
Ruobing LI
;
Liguo CHEN
;
Jing XIAO
;
Huiling GUO
;
Li GUO
;
Chaomin WAN
;
Zhengrong WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2005;9(3):226-228
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate atrial natriuretic peptide(ANP) circadian in the encapsulated human ANP(hANP) cDNA transfected cells,to alter the ANP circadian by artificial control to achieve the objective of effectively treat hypertension or congestive heart failure(CHF). METHODS:ANP cDNA was transfected into Chinese hamster ovary(CHO) cells,which were encapsulated in polycarprolactone(PCL) tubes.The longterm survival of transfected CHO cells and the levels of ANP secreted were detected.Circadian rhythm of ANP secreted by encapsulated transfected cells was also studied,which was regulated by melatonin. RESULTS:During culturing,the ANP level secreted by transfected CHO cells in 2 mL of culture medium within 24 hours could reach 210.3 ng/L in a 20 mm-long and 2 mm-diameter PCL tube.The section of ANP displayed a circadian variation:higher in daytime,but lower at night.The acrophase of circadian rhythm was 4:15 but could be shifted to 7:55 after melatonin management. CONCLUSION:ANP cDNA transfected CHO cells that encapsulated into PCL tube can secret ANP,which might be suitable for the future implantation into human body.Our research provides a new approach in the treatment of hypertension and CHF by ANP.
