Effects of brain-derived neurotrophic factor on healing of tibial fracture of rats
- VernacularTitle:脑源性神经营养因子在大鼠胫骨骨折愈合中的作用
- Author:
Yu QIN
;
Lanxiao WU
;
Guoxian PEI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2005;9(6):202-203
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Recently, there are some researches show that the nutrition and control of nervous system play an important role in the growth of bone and healing of bone fracture.OBJECTIVE: To probe into the effects of brain-derived neurothophic factor on bone fracture healing and its biomechanic features.DESIGN: Randomized controlled study of experiment animals.SETTING: Traumatic orthopaedic and hematology department of a military medical university.MATERIALS: There were 16 SD rats with transverse fracture of shin,which were set up as intramedullary nail internal fixation. They were randomly divided into experiment group and control group with each of 8 mice.INTERVENTIONS: Rats in the experiment group were taken subcutaneous injection of brain derived neurotrophic factor(BDNF) every other day while control group was injected of saline. The shin of mice was collected to observe in the 2nd, 3rd, 4th and 5th weeks.MAIN OUTCOME MEASURES: Gross comparison of fracture healing of each group, biomechanical test and electro microscopic observation.RESULTS: It can be seen by gross observation 2 weeks after operation that the fracture area was linked by connective tissue in both groups and there was obvious movement. In the 3rd week, woven like bone healing could be seen in the experiment group whereas there was obvious fracture end movement in the control group. In the 4th week, bone healing could be seen in both groups, but the bone callus in the experiment group was much smaller with mild angled malformation. In the 5th week, the fracture line in the experiment group already disappeared and the molding of bone fracture was good while there was bigger bone callus in fracture end of the control group. The angle formed by sagittal planes of fracture in the 3rd, 4th and 5th week was(25.00 ± 1.82)°, (24.75 ± 2.50)°, (23.25 ± 3.77)° respectively in the experiment group and(32.00 ±2.45)°, (33.00 ±5.72)°, (29.25± 2.22)° respectively in the control group( P ＜ 0. 05) . The anti-fracture stress in each stage of the experiment group was better than that of the control group( P ＜ 0.05).CONCLUSION: Applying BDNF early and incessantly can stimulate all the stages of bone fracture healing.
