Determination of icariin and adenosine in Cordyceps Vigorine Tablet by HPLC
- VernacularTitle:HPLC测定虫草片中淫羊藿苷和腺苷的含量(英)
- Author:
Qiang MA
;
Yuxin ZHOU
;
Haimin LEI
;
Changhai WANG
- Publication Type:Journal Article
- Keywords:
Cordyceps Vigorine Tablet;
icariin;
adenosine;
HPLC
- From:
Chinese Traditional Patent Medicine
2005;27(6):648-650
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish HPLC for the determination of icariin and adenosine in Cordyceps Vigorine Tablet. METHODS:MetaChem Polaris ODS C18-A column(4.6 mm × 250 mm, 5 μm) was adopted; acetonitrile-water was the mobile phase; detection wavelength was set at 272 nm for icariin and at 260 nm for adenosine; column linearity within the range of 0. 374-2. 246 μg for icariin and 0. 348-1. 392 μg for adenosine, respectively. The average recovery and RSD were found to be (99.4 ± 1.84) % for icariin ( n = 6), (99.63 ± 1.29 ) % for adenosine,respectively. CONCLUSION: The method is convenient, fast, reliable to operate and suitable for the quality control of Cordyceps Vigorine Tablet.
