Effects of Pinacidil preconditioning on hsp70 and cPLA2 expression in hemorrh agic shock rats
- VernacularTitle:吡那地尔预处理对失血性休克大鼠脏器热休克蛋白70和细胞型磷脂酶A2表达的影响
- Author:
Chunhong SHAO
;
Deyao HU
;
Chunsheng XUE
- Publication Type:Journal Article
- From:
Chinese Journal of Trauma
2001;17(4):232-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of Pinacidil prec onditioning protection of hemorrhagic shock rats. Methods The rats were divided into 3 groups: a normal group ( n=10), a control group (n=40) and a preconditioning group (n=40). Pinacidil preconditio ning was processed 24 h before making the hemorrhagic shock model. The cPLA 2 expression in myocardium was observed at different time points with western blot; Pinacidil preconditioning was processed 24, 48 and 72 h before making th e hemorrhagic shock model to observe hsp70 expression in myocardium and liver tissue with western blot. Results Pinacidil preconditioning could increase hsp70 expres sion and decrease cPLA2 expression. Conclusions Pinacidil preconditioning protects "shock cell" by inducing hsp70 overexpression and inhibiting cPLA2 expression, which is re sponsible for protecting myocardial and liver tissues of the hemorrhagic shock rats.
