Application of stochastic resonance to quantitative analysis of weak chromatographic signal of phenazopyridine in human plasma
- VernacularTitle:随机共振应用于人血浆中非那吡啶弱色谱信号的定量分析
- Author:
Yanwei WU
;
Bingren XIANG
;
Erxin SHANG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
stochastic resonance;
phenazopyridine;
weak signal;
HPLC/UV;
HPLC/MS
- From:
Acta Pharmaceutica Sinica
2005;40(7):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Aim To apply stochastic resonance algorithm (SRA) to quantitative analysis of weak chromatographic signal, which was embedded in the noise. Methods Based on the theory of stochastic resonance (SR), a simple and effective SRA has been established to improve analytical detection limits of chromatographic analysis, which apply to enhance the signal to noise ratio by the optimization of the parameters and Runge-Kutta method, was established. The method was used to quantitative analysis of phenazopyridine in human plasma by HPLC/UV. Meanwhile this method is compared with HPLC/MS.Results By experimental chromatographic data sets, an excellent quantitative relationship between concentrations of phenazopyridine and their responses had been obtained. The concentration of phenazopyridine in plasma determined by HPLC/UV with SRA and HPLC/MS showed that there was no significant difference (P ＞ 0.05) between the two methods. Conclusion The new method was feasible.
