Arsenic trioxide-induced HeLa cell death is partially prevented by K + channel blockers
- VernacularTitle:钾通道阻断剂部分抑制三氧化二砷诱导的HeLa细胞死亡
- Author:
Deli DONG
;
Weihua SONG
;
Peilin MA
;
Baofeng YANG
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
HeLa cells;
voltage-dependent K + currents;
4-aminopyridine;
tetraethylammonium
- From:
Acta Pharmaceutica Sinica
2005;40(7):644-648
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of K + channel blockers on arsenic trioxide-induced HeLa cell death. Methods Viability of HeLa cells was assessed by mitochondrial dehydrogenase activity using colorimetric MTT assay and the voltage-dependent K+ currents were recorded by using patch-clamp rest living cells after As2 O3 24 h-incubation showed significant increase of K + currents densities. At + 80mV, the densities of K+ currents (61 ± 18) pA/10 pF (n = 8) in As2O3 24 h-incubation group were significantly more than that in the control group (38 ± 10) pA/10 pF (n = 8, P ＜ 0. 05 ). The HeLa cells were prevented partially from As2 O3-induced cell death by co-application for 24 h with typical voltageeffects on HeLa cells. Conclusion Chronic treatment with As2 O3 increased voltage-dependent K+currents in HeLa cells and the cell death induced by As2O3 was reduced partially by voltage-dependent K +channel blockers, 4-aminopyridine or tetraethylammonium.
