Dual- direction effect of crenulatin on apoptosis of cerebral microvascular endothelial cells and it's mechanism
- VernacularTitle:大花红天素对脑微血管内皮细胞凋亡的双相调节作用及机制
- Author:
Ruizhe QIAN
;
Guoping ZHANG
;
Huiming JIN
;
Wenjian WANG
;
Fei YUE
;
Lianguo SHI
;
Xiaoyi QU
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Crenulation;
bEnd 3 cells
- From:
Chinese Journal of Pathophysiology
2005;21(11):2086-2090
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect and the mechanism of crenulatin, an effective constituent of Chinese traditional medicine, on apoptosis of cerebral microvascular endothelial cells. METHODS: The following terminal concentrations of crenulatin were used in the study: 25 mg/L and 100 mg/L. Apoptosis of mouse cerebral microvascular endothelial cells (bEnd. 3 cell line) was evaluated by flow cytometer, immunocytochemical assay (Fas, Bcl - 2) and Western blotting (caspase - 3) after culture for 24 h. RESULTS: Compared with control group, apoptosis of bEnd. 3 cells in 25 mg/L group was significantly inhibited ( P ＜0.05), but apoptosis in the 100 mg/L group was significantly increased (P ＜ 0.05). In apoptosis inhibited group, the Fas immunocytochemical staining was weaker, the positive cells were significantly decreased ( P ＜ 0.05) and caspase - 3 expression was decreased compared with control group; however, the Bcl - 2 staining was stronger and the positive cells were significantly increased ( P ＜ 0.05). On the other hand, in apoptosis increased group ( 100 mg/L group), the changes were just opposite. CONCLUSIONS: The effect of crenulatin on apoptosis of mouse cerebral microvascular endothelial cells possesses a dual - direction change, inhibitive effect in 25 mg/L and stimulative effect in 100 mg/L group, respectively. The mechanism is related to the alterations of Fas/Bcl - 2 expression and caspase - 3 activity.
