Nuclear factor- κB activation is involved in MCP- 1 expression in human mesangial cells induced by Ox- LDL
- VernacularTitle:核因子-κB活化参与Ox-LDL诱导人肾小球系膜细胞表达单核细胞趋化蛋白-1
- Author:
Huiming WANG
;
Miaozhen ZHU
;
Xiang XU
;
Jianguo ZHANG
;
Yani HE
;
Kailong LI
- Publication Type:Journal Article
- Keywords:
Lipoproteins,LDL;
Mesangial cells;
Monocyte chemoattractant protein- 1;
NF- kappa B
- From:
Chinese Journal of Pathophysiology
2005;21(11):2214-2220
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of nuclear factor- κB (NF- κB) in the expression of monocyte chemoatractant protein- 1 (MCP- 1) in human mesangial cells (HMCs) induced by oxidized low- density lipoprotein (Ox- LDL).METHODS: HMCs were used as target cells. Inhibitory κBα (IκBα) and MCP- 1 protein level was measured by cell ELISA.Activities of transcriptional factors NF- κB were determined by electrophoresis mobility shift assay (EMSA). Immunohistochemistry was used to detect the translocation of Rel p65. RESULTS: NF - κB DNA - binding activation in MCs was observed when 10-100 mg/L Ox - LDL was added to the medium, and 50 mg/L Ox - LDL caused the strongest effect (8.50 ± 1.14, P ＜ 0.01vs control; P ＜ 0.05 vs 10, 25 and 100 mg/L Ox - LDL). The most optimal stimulation time was 60 min ( 11.0 ± 2.11, P ＜0.01 vs control; P ＜ 0.05 vs 30 min or 240 min). IκBα protein level in MC dropped down most obviously after 60 min incubation with 50 mg/L Ox - LDL (0.050 ± 0.006, n = 5, P ＜ 0.01 vs control), while MCP- 1 expression level was the highest (0.331± 0.016, n = 5, P ＜ 0.01 vs control). The translocation of Rel p65 from cytoplasm to nucleus was detected too. NF - κB inhibitor pyrroledithiocarbomate (PDTC) could inhibit these effects induced by Ox- DL. CONCLUSION: Activation of NF- κB regulate the expression of MCP- 1 in HMCs induced by Ox - LDL.
