Influences of wild-type p53 gene overexpression on the differentiation, apoptosis and expression of scavenger receptor CD36 in U937 cells
- VernacularTitle:野生型p53基因导入对U937细胞分化、凋亡和 CD36受体表达的影响
- Author:
Xiangdong YANG
;
Junwen LIU
;
Chun WANG
;
Kai LI
;
Weiqing TANG
;
Hongxia LI
;
Shu WANG
;
Jian LI
- Publication Type:Journal Article
- Keywords:
Monocytes;
Apoptosis;
Antigens,CD36;
Genes,p53;
U937 cells
- From:
Chinese Journal of Pathophysiology
2005;21(9):1752-1757
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of wild-type p53 gene on the differentiation, apoptosis and expression of scavenger receptor CD36 in U937 cells. METHODS: Recombinant adenovirus vector with wild-type p53 gene was constructed and used to transfect U937 cells. With the expression of wild-type p53 gene following adenoviral infection, transfected U937 cells were largely promoted to differentiate into macrophages. RESUITS: Trypanblue-staining test demonstrated that the percentage of positive cells increased from (14.2±5.5)% to (64.6±9.2)% and nitroblue tetrazolium (NBT) reduction test reached similar results (6.3±1.8)% vs (49.7±12.6)%. Furthermore, CD36 mRNA was up-regulated as confirmed by RT-PCR. The increased expression level of CD 36 was also detected by flow cytometry analysis. CONCLUSION: These results suggest that wild-type p53 gene can affect U937 cells differentiation and apoptosis, up-regulate expression of scavenger receptor CD36. It may have a potential significance on atherogenesis.
