Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells
- VernacularTitle:紫杉醇诱导人乳腺癌MCF-7细胞周期阻断及凋亡的基因表达谱分析
- Author:
Jin WANG
;
Fangting HE
;
Chihung TZANG
;
Wangfan FONG
;
Peigen XIAO
;
Rui HAN
;
Mengsu YANG
- Publication Type:Journal Article
- Keywords:
cDNA microarray;
paclitaxel;
gene expression;
MCF-7 cells;
apoptosis
- From:
Acta Pharmaceutica Sinica
2005;40(12):1099-1104
- CountryChina
- Language:Chinese
-
Abstract:
Aim To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. Methods Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9 984 genes and expressed sequence tags (ESTs).Results Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.
