Determination of amodiaquine in human plasma by high performance liquid chromatography
- VernacularTitle:高效液相色谱法测定人血浆中阿莫地喹的浓度
- Author:
Jie ZHANG
;
Qi PEI
;
Shikun LIU
;
Yulu ZHOU
;
Chunshan ZHOU
- Publication Type:Journal Article
- Keywords:
amodiaquine;
chromatography,high pressure liquid;
pharmacokinetics
- From:
Chinese Journal of New Drugs and Clinical Remedies
2005;24(12):925-928
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish HPLC method for the determination of amodiaquine in human plasma.METHODS: Amodiaquine and internal standard(hydroxychloroquine) were analyzed on C18 column(150 mm ×4.6 mm, 5 μm) with methanol: water:triethylamine: orthophosphoric acid (21:77.5:1:0.5 )as mobile phase at the flow rate of 1.0 mL · min-1The UV detector was set at 294 nm. RESULTS: The retention times of amodiaquine and internal standard were 5.82, 8.56 min, respectively. The calibration curve was linear in the range from 10 to 1 000 μg ·L- 1 ( r = 0.999 8, n = 9 ). The limit of quantitation was5 μg· L-1. The extraction recovery was between 75.5 % and 82.7 %, and the methodological recovery was between 97.0 % and 104.8 %. The intra-day and inter-day RSD were less than 6.0 % and 7.5 %, respectively. CONCLUSION: This HPLC method is simple, sensitive and suitable for pharmacokinetic study of amodiaquine.
