Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects.
10.5051/jkape.2009.39.1.77
- Author:
Suk Hoon KWON
1
;
Hyun Chang LIM
;
Kyung Hee CHOI
;
Min Soo KIM
;
Ji Hyun LEE
;
Ui Won JUNG
;
Jeong Ho YUN
;
Chang Sung KIM
;
Seong Ho CHOI
;
Kyoo Sung CHO
Author Information
1. Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University, Korea. kscho@yuhs.ac
- Publication Type:Original Article
- Keywords:
bone morphogenetic protein-2;
E.coli expression system;
Absorbable collagen sponge;
rat calvarial defect
- MeSH:
Animals;
Bone Regeneration;
Collagen;
Durapatite;
Escherichia;
Escherichia coli;
Humans;
Male;
Osteogenesis;
Rats;
Rats, Sprague-Dawley;
Salicylamides
- From:The Journal of the Korean Academy of Periodontology
2009;39(1):77-86
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system. MATERIALS AND METHODS: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals. RESULTS: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks. CONCLUSION: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.
