Molecular identification of Taenia asiatica isolated from a patient in Ha Tay province of Vietnam
- Author:
Nguyen Van De
- Publication Type:Journal Article
- Keywords:
Taenia asiatica
- MeSH:
Taenia;
Centella;
Cestoda;
diagnosis;
Molecular Biology
- From:Journal of Medical and Pharmaceutical Information
2003;10():28-32
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Sample of tapeworm was isolated from 1 patient in Ha Tay province. It was amplified by polymerase chain reaction (PCR), purified, cloned and sequenced. The nucleotide sequence was comparatively aligned with the known corresponding sequences of Taenia asiatica (Taiwanese); T. saginata (Chinese) and T. solium (Chinese and Vietnamese) and used for establishing a phylogenetic relationship for analysis of identification, using special programs. Molecular-based analysis revealed that the Taenia sp from this patient is identified as Taenia asiatica. Absolute nucleotide similarity (100%) between Taenia asiatica in Ha Tay, Vietnam and Taenia asiatica in Taiwan; the grouping together with T. asiatica (Taiwan) confirmed the sample obtained from Ha Tay is closely related to the Taiwanese T. asiatica
