Effect of traditional Chinese herbal preparation Cudong Tongbian capsule on gastrointestinal motility
- VernacularTitle:促动通便胶囊影响胃肠道动力功能的效应
- Author:
Ruiming LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2005;9(27):254-256
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND :Traditional Chinese herbal medicines, according to their different pharmacological effects, may promote, inhibit or bi-directionally regulate gastrointestinal motility. OBJECTIVE:To observe the effect of Cudong Tongbian (CDTB) capsule,a traditional Chinese herbal preparation, on gastrointestinal motility and defecation in rats. DESIGN :Randomized controlled experimental with SD rats. SETTING :Department of Pharmacology of Fenyang College, Shanxi Medical University. MATERIALS:This study was carried out at the Central Laboratory of Fenyang College,Shanxi Medical University between October, 2003 and January, 2004.Fifty SD rats of both gender (by half) with body mass of 200 to 250 g were randomizedinto 5 equal groups, namely normal saline group, high-and low-dose CDTB groups, Bianmitong (BMT) group and glycerol group.Altogether 150 Kunming rats with body mass of 20 to 25 g of both genders (by half) were divided into 3 groups (n=50) for gastric emptying experiment, intestinal movement experiment and defecation experiment, respectively. METHODS:①Gastric emptying experiment of CDTB capsule was performed with domperidoneas the standard control.Fifty SD rats were randomized into 5 groups to receive subcutaneous injection of normal saline (0.2 mL/20 g), solution of CDTB capsule (prepared from the Chinese herbs mainly of costus root, aurantii fructus immaturus, hawthorn fruit, and Rhizoma pinelliae) at high dose (10 g/kg) and low dose (5 g/kg), and domperidone at high dose (30 mg/kg) and low dose (15 mg/kg).Forty minutes later, gastric lavage with 1 g/L methyl orange was performed, 30 minutes after which the rats were killed to obtain gastric tissue rinsed thoroughly with 10 mL distilled water.The optical density of methyl orange in the gastric tissue was determined by colorimetric assay against the optical density of 0.2 mL methyl orange (1g/L) mixed with 0.2 mL 10 mL distilled water.The gastric residual rate =(gastric methyl orange optical density/basic methyl orange absorbence)×100％.②Intestinal movement experiment was carried out with BMT as the standard control.Fifty SD rats were randomized into five groups, namely normal saline group (0.2 mL/20 g), CDTB high dose (10 g/kg) and low dose (5 g/kg) groups, and BMT high-dose (10 g/kg) and low-dose (5 g/kg) groups.CDTB capsule and BMT were dissolved in normal saline for gastric lavage at 0.2 mL/20 g twice a day for 3 consecutive days.Thirty minutes after the last administration, 100 g/L active carbon was used for gastric larvae at the dose of 0.2 mL/20 g.Thirty minutes later, the rats were killed and the mesentery was isolated with the intestines between the pylorus and ileocecal junction taken for examination.The carbon powder movement rate= distance between the pylorus and the farthest carbon powder / total distance between the pylorus and ileocecal junction×100％.③ BMT was used as the standard control in defecation experiment.The grouping and drug administration were similar to those in intestinal moving experiment.The fecal amount within 7 days and the first fecal time after drug administration were recorded, and the fecal wet weight and dry weight(dried at 65 ℃ for 12 hours)were used to calculate fecal water content, and higher water content was thought to facilitate defecation.④ Intestinal water absorbent experiment was performed with BMTand glycerol as the standard controls.After a fast for 24 hours, the rats were anaesthetized by intraperitoneal injection of urethane and the ileocecal junction was ligated, with the intestine above the ileocecal junction ligated into 5 segments where normal saline, glycerol in 0.2 mL normal saline,BMT (10 g/kg), and CDTB at high (10 g/kg) and low doses (5 g/kg) were injected respectively.The rats were killed 2 hours after closure of the incision and edema of each intestinal segment and the intestinal perimeter was observed or measured, with the wet weight and dry weight(dried for 12hours) of the bowel segments determined to calculate the water content. MAIN OUTCOME MEASURS: ① Gastric methyl-organ residual rate,carbon powder moving rate and fecal water content.②Intestinal water content. RESULTS:All 50 rats and 150 Kunming mice survived the experiments without losses.Gastric methyl orange residual rate was significantly lower in high-and low-dose CDTB groups than in the normal saline group [(21.8±6.5)％ and (23.8±7.0)％ vs(36.4±11.1)％, t=3.59, 3.04, P ＜ 0.05)], but similar to that of the high-and low-dose domperidone groups [(19.5 ±5.6)％ ,(22.1±5.6)％,respectively, P ＞ 0.05)].Carbon powder movement rate was significantly higher in high-and low-dose CDTB groups than in the normal saline group [(70.2±3.8)％ and (66.2±2.9)％ vs (52.0±4.1)％, t=10.03, 8.94, P ＜ 0.01)], but similar to high-and low-dose BMT groups [(73.3±3.5)％ and (63.2±2.4)％, respectively, P＞0.05)].Similarly, fecal water content was significantly higher in high- and low-dose CDTB groups than in normal saline group (t=9.51, 7.91, P ＜ 0.01),but similar to high-and low-dose BMT groups (P ＞ 0.05).The intestinal water content was also significantly higher in the two CDTB groups than in normal saline group (t=1 1.13, 6.92, P ＜ 0.05-0.01), but significantly lower than BMT and glycerol groups (t=21.95, 12.18, P ＜ 0.01). CONCLUSION: CDTB capsule, at both high and low doses (10 and 5 g/kg,respectively), can promote gastrointestinal motility and increase fecal and intestinal water content to facilitate gastric emptying and defecation, but higher doses may achieve better effect.Compared with the positive control drugs used in this study, CDTB capsule can increase intestinal water content to a preferable level and cause less side effects.
