Experiment in effect of jiangtang zhongyao No.1 on islet cell secretion in vitro
- VernacularTitle:降糖中药1号对胰岛细胞分泌功能影响的体外实验
- Author:
Ying LIU
;
Zhenfang LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2005;9(43):186-187
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: On bases of preceding study on jiangtang zhongyao No.1 (glucose-reducing No. 1), the experiment was performed in its effect on promotion of insulin secretion of islet cells in vitro.OBJECTIVE: To explore the mechanism of glucose-reducing No.1 so as to provide the scientific evidence for clinical experiment.DESIGN: Neonatal porcine islet cells were collected for generative culture to observe the effect of glucose-reducing No.1 on insulin secretion of islet cells cultured in vitro.SETTING: Department of Pathology of Shandong Province Hospital and Neurological Department of Shangdong Province Hospital of Shandong University.MATERIALS: The experiment was performed in Research Room of Chinese Herbal Pharmacology in Shandong Academy of Medical Sciences, in which, American neonatal pig was selected as the object.METHODS: The obtained neonatal porcine islet cells were randomized into 5 groups, 3 bottles in each group. In culture solution group, the culture solution of equal volume was added. In daonil group, daonil 4 nmol/L was added and in glucose-reducing No.1 0.2 mL, 0.15 mL and 0.1 mL groups, the filter solution of glucose-reducing No.1 0.2 mL, 0.15 mL and 0.1 mL were added successively. The samples in each group were incubated in culture box for 3 hours, and then the upper clear solution was collected to assay insulin content with radioimmunoassay (RIA).MAIN OUTCOME MEASURES: Comparison of active components of islet cells in each group.RESULTS: In basic culture solution, insulin levels were not different significantly among groups. The release amounts in glucose-reducing No. 10.2 mL, 0.15 mL and 0.1 mL groups were increased compared with insulin content in basic culture solution [(784.72±81.25), (462.25 ±14.91);(711.73±91.46), (467.62±15.56); (679.40±78.84), (475.82±13.33) mU/L],and multiple proportion relationship was not obvious with dosage of Chinese herb. In daonil group, insulin secretion was increased significantly [(857.96±77.08), (470.58±15.43) mU/L].CONCLUSION: Jiangtang zhongyao No.1 simulates islet cells to increase significantly the secreted amount of insulin so that the glucose is well reduced.
