Activity Study on Antibacterial Protein Isolated From Musca domestica During Larvae-pupae Metamorphosis
- VernacularTitle:家蝇变形期抗菌蛋白的提取及其抑菌活性研究
- Author:
Xiaohong CAO
;
Zhongli YAN
;
Chunling WANG
- Publication Type:Journal Article
- Keywords:
Musca domestica Metamorphosis Antibacterial protein Antibacterial activity CMsepharose F.F. chromatography
- From:
China Biotechnology
2006;26(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To research the activity of antibacterial proteins isolated from Musca domestica during larvae-pupae metamorphosis. Methods: The 5-day-old larvae of Musca domestica were injured with a needle. 24h later, the larvae which had changed into pupae were selected. The antibacterial protein was isolated by a four-step protocol including grinding, heating, CM-sepharose F. F. cationexchange chromatography and another CM-sepharose F. F. chromatography. Antibacterial activities of samples were tested by an ultrasensitive radial diffusion assay using Staphylococcus aureus as the indicator organism. The molecular weight of the isolated protein was determined by SDS-PAGE.Results: The molecular weight of this isolated protein was 43kDa, and its antibacterial activity was strong. Conclusions: A kind of protein with strong antibacterial activity can be produced in the Musca domestica during larvae-pupae metamorphosis.
