Establishment and characteristics of hybrid embryonic stem cell lines from blastocysts of the (C57BL/6J × 129/J)F1 mouse
- VernacularTitle:C57BL/6J×129/J杂交小鼠ES细胞系的建株
- Author:
Jiaqing ZHANG
;
Weihua YU
;
Xiuming ZHANG
;
Yanwen PENG
;
Weiqiang LI
;
Rui CHEN
;
Xinbing YU
;
Shunong LI
;
Peng XIANG
- Publication Type:Journal Article
- Keywords:
Mice;
Embryonic stem cells;
Hybridization
- From:
Chinese Journal of Pathophysiology
2006;22(1):7-11
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish hybrid mouse embryonic stem (ES) cell line from blastocysts of the (C57BL/6J × 129/J) F1 mouse. METHODS: 3.5 days post- coitus (d.p.c.) blastocysts were cultured on mouse embryonic fibroblasts (MEFs) in the medium, after 3 - 4 days, Inner cell mass were picked up and disaggregated, then reseeded. After the ES - like colonies appeared, passaged them to give permanent ES cell lines. The pluripotent propertes of ES cells obtained were analyzed by alkaline phosphatase (AKP) activity, expression of SSEA- 1 and Oct-4, and their capacity to form teratoma. RESULTS: Two hybrid ES cell lines, SC1001, SC1002 were obtained from blastocysts of the (C57BL/6J × 129/J) F1 genotype. Most of these ES cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines were analyzed on the basis of their alkaline phosphatase activity, expression of SSEA - 1 and Oct - 4, and their capacity to form teratoma in severe combined immunodeficiency (SCID) mice. CONCLUSION: Two hybrid mouse ES cell lines having pluripotent properties and capacity for long - term self renewal were generated from blastocysts of the ( C57BL/6J × 129/J) F1 genotype.
