Neuroprotective effect of taurine against acute cortical neuron injury induced by oxygen-glucose deprivation
- VernacularTitle:牛磺酸对皮质神经元急性氧糖缺失所致损伤的保护作用
- Author:
Can LUO
;
Lianjun GUO
;
Zhikai DAI
;
Qin WU
;
Jingshan SHI
- Publication Type:Journal Article
- Keywords:
taurine;
cerebral cortex;
neurons;
oxygen glucose deprivation;
calcium,cytosolic;
glutamic acid;
apoptosis
- From:
Chinese Journal of Pharmacology and Toxicology
2006;20(1):1-6
- CountryChina
- Language:Chinese
-
Abstract:
AIM Taurine was reported neuroprotective under several ischemic models in vivo. In this study, the direct effect of taurine against oxygen-glucose deprivation (OGD) inducing acute neuronal injury and the underlying mechanisms in vitro were investigated. METHODSFour hours OGD was used to induce in vitro ischemic injury in rat cortical neurons. Taurine 5, 10 and 20 mmol·L-1 was added 20 h before and during 4 h OGD period respectively. Mortality rate of neuron was assayed by MTT and flow cytometry methods. Level of neuronal [Ca2+]i was detected by Fura 2/AM loading. Amino acid concentrations in culture media were measured by high performance liquid chromatography. RESULTS Under OGD conditions, neuronal death was markedly increased, and the levels of neuronal [Ca2+]i and extracellular glutamate level were enhanced obviously. Taurine pretreatment obviously decreased the percentage of neuronal death induced by OGD. In addition, abnormal elevation of neuronal [Ca2+]i and extracellular glutamate level induced by OGD both were markedly repressed by taurine. CONCLUSION Taurine can alleviate rat cortical neuron injury induced by OGD, the mechanisms were likely due to repressing calcium overload and inhibiting excessive release or leakage of glutamate under such conditions.
