Inhibitory effect of triacetylshikimic acid on plasma contents of vasoactive substances and brain myeloperoxidase activity during focal cerebral ischemia-reperfusion in rats
- VernacularTitle:三乙酰莽草酸对大鼠局灶性脑缺血再灌注过程中血浆血管活性物质量和脑髓过氧化物酶活性的抑制作用
- Author:
Xinzhi LI
;
Zhaozhong CHONG
;
Qiuping XU
;
Jianning SUN
;
Jianxun LIU
- Publication Type:Journal Article
- Keywords:
triacetylshikimic acid;
cerebral ischemia;
serotonin;
thromboxane B2;
peroxidase
- From:
Chinese Journal of Pharmacology and Toxicology
2006;20(1):13-18
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore whether plasma vasoactive substances and neutrophil infiltration make different contribution in cerebral ischemia-reperfusion injury and protective effect of triacetylshikimic acid(TSA). METHODSThe rat models of ischemia 90 min and reperfusion 3-48 h were prepared with middle cerebral artery occlusion. TSA 50-200 mg·kg-1 were given (ig) immediately and 60 min again after the onset of ische- mia. Serotonin and thromboxane B2 (TXB2) concentrations in plasma were detected by fluorescence spectrophotometry and radioimmunoassay respectively. Myeloperoxidase(MPO) activity in brain tissue was quantified by chemical analysis. RESULTS At 3-24 h after reperfusion, the concentrations of plasmic serotonin, TXB2, and brain MPO activity increased obviously in a time-dependent manner. At 48 h after reperfusion, the concentrations of serotonin and TXB2 decreased to the same level of sham. Nevertheless, brain MPO activity remained more elevated than the contralateral cortex. At 24 h after reperfusion, TSA (100 and 200 mg·kg-1) was shown to possess the ability to inhibit the increased plasmic serotonin, TXB2 concentrations, and brain MPO activity induced by focal cerebral ischemia-reperfusion. CONCLUSION Vasoactive substances in plasma and MPO activity in brain tissue show different time courses during focal cerebral ischemia-reperfusion and make different contribution to brain damage. TSA is effective to protect the ischemic brain tissue from ischemia-reperfusion injury.
