CHANGES OF EXPRESSION OF CALCITONIN GENE -RELATED PEPTIDE IN THE RAT TRIGEMINAL MOTOR NUCLEUS AFTER MANDIBULAR NERVE TRANSECTION
- VernacularTitle:下颌神经切断术后降钙素基因相关肽在大鼠三叉神经运动核内表达的变化
- Author:
Tao ZHANG
;
Youwang PANG
;
Fuxing ZHANG
;
Tao LIU
;
Yulin DONG
;
Jinlian LI
- Publication Type:Journal Article
- Keywords:
calcitonin gene-related peptide;
astrocyte;
trigeminal motor nucleus;
peripheral nerve injury;
immunohistochemistry
- From:
Chinese Journal of Neuroanatomy
2006;22(2):123-129
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the changes of calcitonin gene-related peptide (CGRP) expression in the trigeminal motor nucleus (Vmo) following mandibular nerve transection. The expression of CGRP and glial fibrillary acidic protein (GFAP) were detected at 3, 7, 14, 21, 28, 35 and 42 days post-axotomy (dpa) by using immunohistochemical and double-immunofluorescence histochemical methods. And the number of CGRP-positive surviving motoneurons was examined by using image analysis system. The present results showed that there was a "biphasic" up-regulation of CGRP-immunoreactivity at 3 and 35 dpa on the ipsilateral side of the Vmo, and significant changes were also observed on the contralateral side. Quantitative analysis revealed that there was almost no loss of motoneurons in the Vmo but there was an evident increase in GFAP expression around the Vmo neurons on the ipsilateral side after axotomy. The present results suggest that the up-regulation of CGRP may exert its trophic effects in both the acute stress reaction and the recovery of the injured motoneurons, and the activated astrocytes may be well involved in this process.
