Effect of ginkgolide B on the platelet-activating factor induced changes of chemotaxis and cytoskeleton of macrophages
- VernacularTitle:银杏内酯B对血小板活化因子引起的巨噬细胞趋化及细胞骨架改变的影响
- Author:
Shanying PENG
;
Fuying ZHANG
;
Xueyu OUYANG
;
Yang LIU
;
Wenjie WANG
- Publication Type:Journal Article
- Keywords:
chemotaxis;
F-actin;
Ca2 +;
peritoneal macrophages;
ginkgolide B
- From:
Acta Pharmaceutica Sinica
2006;41(2):156-160
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the inhibitory effect of ginkgolide B (BN52021) on the PAF induced changes of chemotaxis of murine peritoneal macrophages and the related polymerization of F-actin.Methods Chemotaxis assays were performed using a modified 48-well Boyden chamber. Actin polymerization of murine peritoneal macrophages was analyzed by flow cytometry using a specific fluorescent stain. Results Peritoneal macrophages significantly migrated toward platelet-activating factor(PAF) through a micropore filter; however, in the presence of PAF receptor antagonist BN52021 (0. 01the actin polymerization of murine peritoneal macrophages induced by PAF in the presence of Ca2+ , but not in Ca2+ -free medium. Conclusion The results suggested that preventing polymerization of F-actin may be a pathway by BN52021 to inhibit the chemotaxis of macrophages, and this effect seems to be Ca2+dependent. The data further indicated that inhibition of PAF induced macrophage chemotaxis is an important mechanism underlying the anti-inflammatory action of BN52021.
