MIGRATION AND DIFFERENTIATION OF NSCs TRANSPLANTED INTO ADULT RAT HIPPOCAMPUS DAMAGED BY KAINIC ACID
- VernacularTitle:神经干细胞在海人酸毁损大鼠海马中的迁移和分化
- Author:
Xing WU
;
Peiyun ZHANG
;
Li LUO
;
Changfen XU
- Publication Type:Journal Article
- Keywords:
neural stem cells;
migration;
cell differentiation;
hippocampus
- From:
Chinese Journal of Neuroanatomy
2006;22(3):245-252
- CountryChina
- Language:Chinese
-
Abstract:
The present study aims to investigate the survival, migration and differentiation of the neural stem cells (NSCs) transplanted into the hippocampus of adult rat damaged by kainic acid. Hippocampal CA1 pyramid neurons were degenerated by stereotaxical injection of kainic acid, one week later, NSCs labeled by Hoechst33342 were transplanted into the damaged hippocampus. The rats were sacrificed at 1,2,4 and 8 weeks, and the brains were examined by immunohistochemical analysis to observe the survival, migration and differentiation of the NSCs in the lesioned hippocampus. The results showed that transplanted neural stem cells migrate in the mode of chain in pyramid layer of hippocampus and most of them express the astrocytic marker of GFAP, others express the neuronal marker of MAP2. These results suggest that transplanted neural stem cells migrate in the mode of chain in pyramid layer of hippocampus. Most of them differentiate intoastrocytes, others differentiate into neurons.
