Effect of captopril on prolongation of action potential duration and reduction Ik in ischemia pig ventricular myocytes
- VernacularTitle:卡托普利对豚鼠心室细胞动作电位及外向延迟整流钾电流的作用
- Author:
Yanmin XU
;
Tigang HUANG
;
Yuanlu CHEN
- Publication Type:Journal Article
- Keywords:
Patch- clamp techniques;
Captopril;
Action potentials;
Outward rectifier potassium current;
Myocytes
- From:
Chinese Journal of Pathophysiology
2006;22(3):456-459
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the effect of captopril on action potential duration and outward delayed rectification potassium current (Ik). METHODS: Action potentials were recorded using a conventional glass microelectrode filled with 3 mol/L KCl solution. Membrane patch clamp whole cell recording technique was used to investigate the Ik current maximum in the holding potential - 50 mV, lasting time 100 ms, command potential + 40 mV. RESULTS: The action potential duration of 30%, 50% repolarization (APD30, APD50) and ERP were significantly prolonged, but APD90 wasn' t prolonged significantly when captopril group compared with ischemic group. The amplitude of Ik increased significantly in ischemic group, but significantly decreased in captopril group and in captopril + ischemic group. The shapes of current - voltage relationship were unchanged among groups, but significantly upward in ischemic group and downward in captopril and captopril + ischemic group. CONCLUSION:Captopril exerts electrophysiologic action due to decreasing delay outward rectification potassium current and prolonging action potential duration of APD30, APD50 and ERP.
