Quantitative detection of cyclooxygenase - 2 gene expression in carcinoma of larynx by real- time polymerase chain reaction
- VernacularTitle:喉癌组织中环氧化酶- 2(COX-2)基因表达的实时定量PCR研究
- Author:
Shusheng GONG
;
Juan DING
;
Qing CHANG
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase - 2;
Laryngeal neoplasms;
Fluorescent dyes;
Gene expression
- From:
Chinese Journal of Pathophysiology
2006;22(3):501-505
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To quantify cyclooxygenase - 2 (COX - 2) mRNA in carcinoma of larynx and evaluate the correlation between the quantity of COX - 2 mRNA and clinical staging or histological grade. METHODS: The expression of COX - 2mRNA in 30 cases of carcinoma of larynx tissue and adjacent non - cancerous tissues were evaluated by PCR, which includes a fluorescence dye , SYBR green Ⅰ , and the sequence specific primer. The GAPDH was used as control. RESULTS: The specificity of products was proved to be COX - 2 and GAPDH by the analysis of the melting curve of the amplified products and agarose gel electrophoresis. The expression of COX - 2 mRNA was detected in all cancerous tissues of 30 patients (100%), but only in 12 adjacent non - cancerous tissues of 30 patients (40%). The NCOX value of carcinoma of larynx tissue and adjacent non - cancerous tissues was 16.54 ± 13.27 and 9.24 ± 6.91, respectively, and the expression levels of COX- 2 mRNA elevated significantly in laryngeal squamous cell carcinoma tissue and there were significant correlation between the expression levels of COX - 2mRNA and clinical stage or histological grade. CONCLUSION: The expression of COX - 2 mRNA in carcinoma of larynx can be determined by real - time PCR technique. An increase in COX - 2 mRNA may be associated with carcinogenesis of carcinoma of larynx, and it may be useful as a biomarker in laryngeal cancer.
