Apoptosis of human hepatoma cell lines induced by transforming growth factor beta 1 (TGF-β1) correlates with p53 and Smad4 activation
- VernacularTitle:转化生长因子β1(TGF-β1)诱导人肝癌细胞系的凋亡与p53及Smad4活化相关
- Author:
Chunlei WANG
;
Yuanlian WAN
;
Yucun LIU
;
Zhiqiang HUANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
Apoptosis;
Liver neoplasms;
Cell line;
Signal transduction
- From:
Journal of Peking University(Health Sciences)
2006;38(2):176-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the relationships between apoptosis induced by transforming growth factor beta 1 (TGF-β1) and Smad in human hepatoma cell lines. Methods: Three human hepatic carcinoma cell lines, involving different status of the p53 gene respectively, were used in this study.TGF-β1-induced apoptosis in hepatic carcinoma cell lines was quantitated using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. For identification of the mechanism of apoptosis induced by TGF-β1, these cell lines were transfected with a TGF-β1-inducible luciferase reporter plasmid containing Smad binding elements (SBE) and luciferase gene using LF2000, then were treated with TGF-β1. Relative luciferase activity was assayed respectively. Results: Among three cell lines studied with TUNEL assay, addition of TGF-β1 induced apoptosis only in HepG2 cells (wild type p53). In contrast, Huh-7 ( mutant p53) and Hep3B ( deleted p53) cell lines lacked apoptosis. The detection of luciferase activity indicated that HepG2 cells dramatically increased the response to TGF-β1 induction, Huh-7 and Hep3B cell lines significantly lowered luciferase expression. Conclusion: HepG2cells were highly susceptible to TGF-β1-induced apoptosis compared with Hep3B and Huh-7 cell lines.Smad4 may be a central mediator of the TGF-β1 signaling transdution pathway.
