OsCOI1, a Putative COI1 in Rice, Show MeJA and ABA Dependent Expression
- VernacularTitle:OsCOI1,水稻中一个受茉莉酸甲酯和脱落酸诱导表达的F-box家族基因
- Author:
Tingzhang HU
;
Weiping WANG
;
Kaiming CAO
;
Xiping WANG
- Publication Type:Journal Article
- Keywords:
rice;
F-box motif;
semi-quantitative RT-PCR;
Northern blot;
JA signal pathway;
ABA pathway
- From:
Progress in Biochemistry and Biophysics
2006;33(4):388-393
- CountryChina
- Language:Chinese
-
Abstract:
A novel gene, which was a homologue of Arabidopsis COI1 was isolated from rice (Oryza sativa L.) by RT-PCR and designated as OsCOI1. It encoded a protein of 595 amino acids. The similar F-box motif and 16 leucine-rich repeats were found in the deduced protein OsCOI1. OsCOI1 and COI1 showed high homology (74%) at amino acid level. Semi-quantitative RT-PCR and Northern blot analysis demonstrated that the expression of OsCOI1 in rice varied obviously after treatment with MeJA and ABA but was not affected by SA and ET, suggesting that the specific function of OsCOI1 in JA signal pathway and related ABA pathway.
