(-)-Epigallocatechin-3-gallate suppresses migration and invasion of cancer cell by decreasing expression of small GTP-binding protein RhoA
- VernacularTitle:表没食子儿茶素没食子酸酯通过下调GTP结合蛋白RhoA的表达抑制癌细胞的浸润和转移
- Author:
Jingya YANG
;
Xichang WANG
;
Meifang LIU
;
Bailin LI
- Publication Type:Journal Article
- Keywords:
(-)-Epigallocatechin-3-gallate;
cancer;
RhoA;
migration;
invasion
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2006;11(7):729-734
- CountryChina
- Language:Chinese
-
Abstract:
AIM: It is tested that the suppressive effects of (-)-Epigallocatechin-3-gallate (EGCG ) on the migration, invasion and RhoA expression of human lung carcinoma 95-D cells and B16BL6 melanoma cells invasion in vivo,which will possibly help to understand the molecular mechanisms by which EGCG inhibits the invasion of tumor cells. METHODS: The inhibitory effect of EGCG on the migration of 95-D cells was tested by cell migration assay. Cell invasion was analyzed by the matrigel invasion assay. Assay of tumor metastasis in an animal model, RT-PCR and Western blot analysis of expression of RhoA was also performed. RESULTS:EGCG was effective in inhibiting the migration of 95-D cells in a dose-dependent manner. EGCG dose-dependently inhibited 95-D cells invasion in vitro and 40 μmol·L-1 EGCG exhibited 79.9% inhibition and EGCG 50 mg·kg-1·d-1 for 3 weeks inhibited B16BL6 melanoma cells invasion by 71.7% in vivo. EGCG could down-regulate the expression of RhoA. CONCLUSION: EGCG strongly inhibits metastasis of 95-D cells, and the mechanism of EGCG is possible associated with the inhibition of RhoA expression.
