NEUROTOXIC EFFECT OF HIV gp120 ON CULTURED RAT DORSAL ROOT GANGLION NEURONS
- VernacularTitle:人类免疫缺陷病毒蛋白HIV gp120对培养的大鼠背根神经节神经元的神经毒性作用
- Author:
Huaxiang LIU
;
Zhenzhong LI
;
Yi XING
;
Fei HUANG
;
Fang DU
;
Zhen LIU
;
Shuyan CHEN
;
Lihong WANG
;
Huaijing WANG
- Publication Type:Journal Article
- Keywords:
human immunodeficiency virus;
HIV gp120;
neuron;
dorsal root ganglia;
rat
- From:
Chinese Journal of Neuroanatomy
2006;22(6):603-608
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the neurotoxic effect of human immunodeficiency virus (HIV) gp120 on cultured dorsal root ganglion (DRG)neurons in vitro, dissociated and organotypic mouse embryo's DRG cell culture models were established. Both dissociated and organotypic DRG cultures were treated with HIV gp120 in different concentration (250 pmol/L and 1 nmol/L, respectively, 2 times/7 days). For dissociated DRG cultural cells, microtubule-associated protein 2 (MAP2) immunofluorescent labeling was processed for observing the changes of neuronal cell body and neurites. The change of the ultrastructure in the organotypic cultured DRG was observed by electron microscopy.The difference of the number and length of neurites between the control group and HIV gp120 treated groups were significant (P＜0.001),whereas there was no significant difference in the diameter of neurons between them (P＞0.05). The ultrastructural changes included the decrease or loss of cristae in mitochondria and accumulation of many high densed particles between the microtubules and the neurofilaments by using both the concentrations of HIV gp120 treatment. The present results indicate that HIV gp120 had a directly neurotoxic effect on the cultured DRG neurons, especially more sensitive to mitochondria.
