Inhibitory kinetics of paeonol on the activity of mushroom tyrosinase oxidizing L-Dopa
- VernacularTitle:丹皮酚抑制蘑菇酪氨酸酶催化氧化L-Dopa的动力学
- Author:
Shengzhao GONG
;
Jiang CHENG
;
Zhuoru YANG
- Publication Type:Journal Article
- Keywords:
tyrosinase;
paeonol;
kinetics;
inhibitor
- From:
Acta Pharmaceutica Sinica
2006;41(6):561-564
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effect of paeonol on the activity of tyrosinase and provide experimental evidence for the treatment of hyperpigmentation disorders. Methods Tyrosinase activity was estimated by measuring the oxidation rate of L-3,4-dihydroxyphenylalanine (L-Dopa). The inhibitory effects of paeonol on the activity of mushroom tyrosinase and Michaelis-Menten kinetics were deduced from the Lineweaver-Burk plots. Results The inhibitory concentration of paeonol leading to 50% enzyme Paeonol is a potential mixed inhibitor of mushroom tyrosinase. The mixed inhibition function may originate from its ability to form a Schiff base with a primary amino group and to chelate copper at the active site of tyrosinase.
