Sodium caffeate induces endothelial cell apoptosis and inhibits VEGF expression in cancer cells
- VernacularTitle:咖啡酸钠诱导内皮细胞凋亡及抑制癌细胞VEGF的表达
- Author:
Xu FENG
;
Ouyang ZHIGANG
;
Zhang SHENGHUA
;
Song DANQING
;
Shao RONGGUANG
;
Zhen YONGSU
- Publication Type:Journal Article
- Keywords:
apoptosis;
endothelial cell;
microenvironment;
sodium caffeate;
VEGF
- From:
Acta Pharmaceutica Sinica
2006;41(6):572-576
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the induction of endothelial cell apoptosis and the suppression of VEGF expression in cancer cells by sodium caffeate (SCA). Methods Apoptosis of transformed human umbilical vein endothelial cells (ECV304 cell line) was detected by flow cytometry, DNA electrophoresis assay and morphological assessment. Western blotting analysis was applied for determination of VEGF expression in cancer cells. Substrate degradation by type Ⅳ collagenase was measured by zymography.ELISA was used to detect the binding of type Ⅳ collagenase with relevant monoclonal antibody. Results SCA induced ECV304 cell apoptosis in a time- and dose-dependent manner. After treatment with 100 and fluorescence and distinct changes of nuclear morphology, such as pyknosis and the occurrence of apoptotic bodies. VEGF expression in hepatoma HepG-2 cells and prostate carcinoma DU145 cells was reduced after SCA treatment. The degradation activity of type Ⅳ collagenase including MMP-2 and MMP-9 secreted by giant cell pulmonary carcinoma PG cells was inhibited by SCA in a dose-dependent manner. SCA also reduced the binding of mAb 3D6, a relevant monoclonal antibody, to type Ⅳ collagenase. Conclusion SCA can induce endothelial cell apoptosis and inhibit VEGF expression as well as type Ⅳ collagenase activity in cancer cells. SCA might be active in modulating tumor angiogenesis and the microenvironment.
