Molecular Cloning and Characterization of a Putative Promoter Region of mPC-1 Gene Homologous to hPC-1
- VernacularTitle:与人PC-1同源的鼠PC-1基因的启动子的分子克隆和特性分析
- Author:
Ruixia LIANG
;
Zhijie TU
;
Jian WANG
;
Hui ZHANG
;
Fei JIANG
;
Bo PANG
;
Bin ZHENG
;
Suping LI
;
Qingguo SHI
;
Cuifen HUANG
;
Jianguang ZHOU
- Publication Type:Journal Article
- Keywords:
mPC-1 gene;
promoter;
prostate cancer;
LNCaP cell line
- From:
Chinese Journal of Biochemistry and Molecular Biology
2006;22(11):856-861
- CountryChina
- Language:Chinese
-
Abstract:
To identify the regulatory region that are responsible for the expression of mPC-1, we have isolated and characterized the mPC-1 gene promoter. Sequence analysis of the mPC-1 5' -flanking region and a series of truncated constructs were performed, which were transiently transfected into the prostate cancer cell lines and non-prostate cancer cell lines and analyzed through Dual-luciferase reporter assay system. The relative activity of mPC-1 gene promoter was by far higher than pGL3-control containing SV40 promoter and enhancer and p61-PSA containing hPSA 6 kb promoter in AR (androgen receptor, AR ) -positive prostate cancer cell lines. The region from 599 bp to 449 bp of mPC-1 promoter might contain a negative regulatory element. The expression of mPC-1 1.1 kb fragment is mainly restricted into prostate cancer cell lines. The relative activity of mPC-1 1.1 kb 5'-flanking region was regulated by androgen. The results demonstrated that the 1.1 kb fragment of mPC-1 5' -flanking region was relatively strong and prostate cancer cell specific promoter region.The 1.1 kb promoter of mPC-1 gene might be well suited to prostate cancer gene therapy if the promoter was properly modified.
