Detergent Resistant Microdomains at Cardiac Sarcoplasmic Reticulum Membranes
- VernacularTitle:心肌肌浆网膜含有膜质微区结构
- Author:
Ling WANG
;
Cunfu LU
;
Xujia ZHANG
- Publication Type:Journal Article
- Keywords:
sarcoplasmic reticulum;
Ca2+-ATPase;
microdomain
- From:
Progress in Biochemistry and Biophysics
2006;33(9):877-880
- CountryChina
- Language:Chinese
-
Abstract:
It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here the isolation of microdomains from porcine cardiac sarcoplasmic reticulum (SR) membranes is described. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Both ganglioside GM1 and caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane are present in the SR-DRMs. It was demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs, and are fully functional. The results suggested that SR membranes like other biomembranes,such as the plasma membrane, contained microdomains.
