Fusion Immunotoxin Anti-HER-2-scFv-SEC2 Expressed in E.coli With an Improved Expression Vector pASK75-EX: Its Construction and Functioning
- VernacularTitle:抗Her-2-scFv-SEC2融合免疫毒素的构建和功能研究
- Author:
Mingkai XU
;
Chenggang ZHANG
;
Huiwen ZHANG
;
Yafeng ZHOU
;
Xianen ZHANG
;
Li LIU
- Publication Type:Journal Article
- Keywords:
staphylococcal enterotoxin C2 (SEC2);
HER-2;
fusion immunotoxin;
tumor- targeting;
pASK75
- From:
Progress in Biochemistry and Biophysics
2006;33(8):781-788
- CountryChina
- Language:Chinese
-
Abstract:
A tumor-targeting recombinant fusion immunotoxin B-L-SEC2 was constructed by fusing staphylococcal enterotoxin C2 (SEC2) and an anti-HER-2 single-chain Fv B1 through a peptide linker, and expressed in E. coli strain BL21 (DE3) with an improved expression vector pASK75-EX as inclusion body. The denatured inclusion body was purified with Ni-NTA chelate agarose, and then re-natured by dialysis. FACS and MTT assays indicated that the re-natured fusion immunotoxin B-L-SEC2 could target the HER-2 over-expressing breast tumor cell SK-Br-3 in vitro, and inhibit the growth of SK-Br-3.
