Human bone marrow mesenchymal stem cells differentiate toward endothelial lineage in vitro
- VernacularTitle:人骨髓间充质干细胞体外内皮细胞诱导分化的研究
- Author:
Xin GUO
;
Yulin LI
;
Xu HE
;
Wei LI
;
Yilei LI
;
Lihong ZHANG
;
Ruiguang YANG
;
Wei ZHAO
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Endothelial cells;
Endothelial growth factors;
Fibronec-tins;
Collagen type I
- From:
Chinese Journal of Pathophysiology
2006;22(8):1586-1590
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the cytological basis and differentiating conditions of human bone marrow mesenchymal stem cells (hMSCs) differentiated into cells of the endothelial lineage in vitro. METHODS: hMSCs were isolated by density gradient centrifugation and fractionated on a 1 073 g/L Percoll. The combination of VEGF165 and various matrix proteins including fibronectin (FN) and type I collagen (Col) was used to induce hMSCs in vitro. Cells were characterized by immunohistochemistry, cytochemistry, FACS and ultrastructure to identify and detect the differentiated population and markers. RESULTS: hMSCs was positive for KDR. PAS reaction was positive and ultrastructure of hMSCs showed glycogen- pool in ectoplasm. Glycogen reducing or disappear suggested that stem cells have occurred differentiation. Induction of hMSCs resulted in the increase of KDR, β1 integrin and CD34. CONCLUSION: hMSCs were induced to a transit population (TP( )) that differentiated toward the endothelial progenitor cells ( EPC), but not a really EPC. hMSCs pedigree diagram of differentiation was hMSCs→TP→EPC→endothelial cells (Ecs).
