Analgesia of allied fuzi tang and shaoyao gancao tang and its approach
- VernacularTitle:附子汤合用芍药甘草汤镇痛作用效果及其途径
- Author:
Ruiming LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2006;10(7):180-182
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: Analgesic applied at present induces harmful effects of different ways and degrees, which restricts the treatment of pain to a certain extend. Analgesia of Chinese drug with multi-target and multi-layer mechanism is advantageous to the treatment of pain.OBJECTIVE: To observe the analgesia of allied fuzi tang and shaoyao gancao tang (allied decoction) and its approach on painful animal model.DESIGN: Randomized controlled observation was designed.SETTING: Center Laboratory Room of Fenyang College of Shanxi University of Medical Science.MATERIALS: The experiment was performed in Center Laboratory Room of Fenyang College of Shanxi University of Medical Science from October to December 2004. Totally 50 SD rats and 60 Kunming mice were employed. By pre-screening of pain threshold, the animals with similar threshold were selected in the experiment.METHODS:① Preparation of allied decoction:fuzi (Radix Aconiti Lateralis Preparata) was decocted firstly for 1 h, shaoyao (Paeonia Lactiflra Pall.), baishao (Radix Paeoniae Alba.) (stirred-fried with wine) and gancao (Radix Glycyrrhizae) (prepared with honey) were extracted with 750 mL/L ethanol successively, 0.5 hour/time and the ethanol was recycled totally for 3 times. The extracts were collected together and concentrated as 4 g/mL of raw herbs.② Analgesia of allied decoction on painful rats induced by formaldehyde solution: 50 SD rats were divided into 5 groups, named model group, aspirin group and allied-decoction groups of 40 g/kg, 20 g/kg and 10 g/kg successively, 10 rats in each one. In model group and aspirin group, formaldehyde solution 25 mL/L and aspirin 0.2 g/kg were applied respectively. Gastric perfusion was applied for medication in each group.Before the medication, in every group, 25 mL/L formaldehyde solution 50 μL was injected subcutaneously on dorsum of left hindquarter of rat. Cumulative time of pain reaction in 1-5 minutes ( Ⅰ phase) and 15-40 minutes (Ⅱ phase) was recorded respectively after injection for evaluation.Painful integral= (time of raising the injected hindquarter ×1+ time of biting the trembled injected hindquarter ×2)/300. After bred for 1 week,the experimental animals were medicated by gastric perfusion continuously for 3 days. On the 3rd day, 1 hour after medication, 25 mL/L formaldehyde solution 50 mL was injected subcutaneously on dorsum of left hindquarter.Painful integral was calculated based on the above method. Analgesic score =integral after medication/integral before medication ×100％.③ Determination of nitrogen monoxide (NO), prostaglandin (PG) and superoxide dismutase (SOD) in serum and spinal cord in painful mice induced by glacial acetic acid: 60 kunming mice were divided into 6 groups, named blank control, model group, aspirin group and allied-decoction groups of 40 g/kg, 20 g/kg and 10 g/kg successively, 10 mice in each one. In blank control and model group, physiological saline 10 mL/kg and 6 g/L glacial acetic acid 10 mL/kg were injected abdominally respectively. In aspirin group and allied-decoction groups of 40 g/kg, 20 g/kg and 10 g/kg successively, 1 hour after medication on the 3rd day, 6 g/L glacial acetic acid 10 mL/kg was injected abdominally. Ten minutes after the injection, blood was collected from posterior ocular venous plexus. Serum was separated to determine NO concentration (catalytic spectrophotometry), PG content (UV spectrophotometry) and SOD activity.MAIN OUTCOME MEASURES:① Analgesic integralof rats in each group.② Determination of NO,PG contents and SOD activity in serumand spinal cord in serum and spinal cord in mice.RESULTS: Totally 50 SD rats and 60 kunming mice were all in result analysis,no dropped-out case.① Comparison of analgesic integrals ofrats in each group: Analgesic integral of Ⅰ phase in allied-decoction group of 40 g/kg was lower significantly than that in model group (82.1±9.8, 95.3±8.7, t=3.17, P ＜ 0.05). The integrals of allied-decoction groups of 40 g/kg and 20 g/kg at Ⅱ phase were lower significantly than those in model group (69.7±10.4, 73.2±7.5, 98.9±6.7, t=6.64, 6.08, P ＜ 0.01).② Comparison of NO,PG contents and SOD activity in serum and spinalcord in mice in each group: Compared with model group, NO and PG contents in aspirin group and allied groups of 40 g/kg, 20 g/kg and 10 g/kg were reduced significantly and SOD activity was increased significantly (t=3.21-19.30, P ＜ 0.05-0.01). Compared with aspirin group, NO and PG contents in allied decoction group of 40 g/kg and 20 g/kg were re duced significantly and SOD activity was increased significantly (t=2.82-7.43, P ＜ 0.05).CONCLUSION: Allied decoction inhibits pain at Ⅰ and Ⅱ phases induced by formaldehyde solution, significantly reduces NO and PG contents in serum and spinal cord of painful mice induced by glacial acetic acid and increases SOD activity. It is suggested that the alliance of two formulas provides analgesia of central nerve and peripheral nerve ending, which is probably associated with the alternations of NO, PG and SOD.
