Implantation study on the potential of murine epidermal stem cell differentiation
- VernacularTitle:小鼠表皮干细胞分化潜能的体内植入研究
- Author:
Yi LIN
;
Haibiao LI
;
Jintao HUANG
;
Shan ZENG
;
Jingfang DI
;
Yaoying ZENG
- Publication Type:Journal Article
- Keywords:
Cell surface marker;
Epidermis;
Stem cells
- From:
Chinese Journal of Pathophysiology
2006;22(12):2418-2423
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the potential of murine epidermal stem cell (ESC) differentiation after seeded in a biodegradable carrier and implanted subcutaneously into syngeneic recipient mice. METHODS: ES cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a polyglycolic acid (PGA) net containing collagen gel, functioning as a cell carrier, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. RESULTS: The ESCs kept alive in the implant when observed under a fluorescent microscopy 3 weeks or longer after implantation, and could differentiate into hair follicle - like structure,glandular structure, and gave rise to additional structures displaying features resembling native dermis. No apparent rejection or severe side effects were observed at least 10 weeks post- implantation. CONCLUSION: It is feasible to use these ESCs as seed cells in the study to fabricate dermal equivalent having the potential to develop dermal appendages.
