Effect of two modified compound preparations of wuling san on blood brain barrier
- VernacularTitle:传统方剂五苓散加减后的2种复方制剂对血脑屏障保护作用比较
- Author:
Tai LIU
;
Yuping TANG
;
Xiangfa ZENG
;
Jing Lü
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2006;10(15):168-170
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: On the basis of traditional herb wuling san, tianqi and danshen are added to make a preparation, jianshen lishui I , for treating blood stasis and edema; shichangpu and xiakucao are added to make a preparation, xingnao xiaozhong capsule, for treating fire, sputum and qiaobi in cerebral edema.OBJECTIVE: To observe the differences between jianshen lishui Ⅰ and xingano xiaozhong capsule on treating vascular-derived cerebral edema.DESIGN: Randomized grouping design and blank controlled study.SETTING: Laboratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine.MATERIALS: The experiment was completed in the Lab.oratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine from March to April 2001. Totally 32 male rabbits with 7 months old were selected in the study.METHODS: ① Animal models with cerebral edema were established with self-fresh blood injection method. According to grouping method, animals were divided randomly into 4 groups: blank control group (without intervention); model group (without intervention after modeling); jianshen lishuiⅠ group (11.5 mL/kgjianshen lishui Ⅰ was perfused after modeling; jian-shen lishui Ⅰ consisted of 15 g sanqi, 15 g danshen, 10 gfuling, 10 gzhuling, 10 g zexie, 10 g baizhu and 10 g gu~hi, and provided by Dispensary of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine, 200 mL/bag) and xingnao xiaozhong capsule group [after modeling, 11.5 mL/kg xingnao xiaozhong suspension was made by 380 mg/kg boiled distilled water; certification: southern medicine (96) 05079; consisting of 15g xiakucao, 15 g shichangpu, 10 gfuling, 10 g zhuling, 10 g zexie, 10 g baizhu and 10 g guizhi, 13.33 g raw materials per capsule; provided by Agent Department of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine] with 8 in each group. Animals in each group were divided into 12 and 24 hours subgroups with 4 in each time points. ② Animals were killed 12 and 24 hours after intervention with air embolism to calculate cerebral coefficient (total brain mass/body mass × 100%), cerebral water content [(wet weight - dry weight)/wet weight × 100%], Evens blue content in brain tissue [absorbency (A value) × microgram in standard liquid/an A value × (25 mL/wet weight)]. ③Positivity was tested with square method; differences among groups were compared with F test; differences between groups were compared with q test; time points were tested with t test; and irregular variance was checked with rank sum test.MAIN OUTCOME MEASURES: Comparisons of cerebral coefficient,water content and content of Evans blue in 12 and 24-hour experiment group.RESULTS: Totally 32 rabbits entered the final analysis. ① 12-hour experiment: Water content and Evans blue content in jianshen lishui Ⅰgroup and xingnao xiaozhong capsule group were obviously lower than those in model group (P ＜ 0.01); cerebral coefficient in jianshen lishui I group was lower than that in xingnao xiaozhong capsule group, but the difference was not significant (P ＞ 0.05); water content and Evans blue content in jianshen lishui Ⅰ group were lower than those in xingnao xiaozhong capsule group (P ＜ 0.05). ② 24-hour experiment: Water content and Evans blue content in jianshen lishui Ⅰ group and xingnao xiaozhong capsule group were obviously lower than those in model group (P ＜ 0.01);cerebral coefficient in jianshen lishui Ⅰ group was lower than that in model group (P ＜ 0.01); cerebral coefficient, water content and Evans blue content in jianshen lishui Ⅰ group were respectively lower than those in xingnao xiaozhong capsule group (P ＜ 0.05).CONCLUSION: Vascular-derived edema has found 12 hours after modeling and cerebral edema deteriorates with time passing. In addition, effect of jianshen lishui Ⅰ on blood brain barrier is superior to that of xingnao xiaozhong capsule.
