Study on immune response of antibodies IgA1 and IgG fron sera of hepatoma and acute leukaemia patients by lectin-ELISA and lectin-protein A
- Author:
Do Ngoc Lien
- Publication Type:Journal Article
- Keywords:
hepatoma and acute leukaemia
- MeSH:
Carcinoma, Hepatocellular;
Enzyme-Linked Immunosorbent Assay;
Leukemia;
- From:Journal of Medical and Pharmaceutical Information
2003;9():23-25
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Healthy human serum IgA, purified lectin-sepharose 4B column affinity chromatography was assessed by sodium dodecyl sulfate polyacrylamide electrophresis. The lectin from wild Jackfruit reacted specifically to IgA1 from human serum. This lectin used as antigen in the technique of ELISA-Lectin to determine the concentration of IgA1 from healthy and pathological human sera. Using protein A from Staphylococcus aureus isolated from Vietnamese infectious patients to capture IgG average concentration of IgA1 from healthy human serum as 14.37 mg/ml. No change of IgG concentration from patients sera (hepatoma and leukemia) was obtained in comparison with healthy subjects
