Analysis on the level of metallic elements in traditional Chinese medicine for treatment of cerebrovascular disease
- VernacularTitle:治疗脑血管疾病中药金属元素的含量分析
- Author:
Qinghua ZHAO
;
Siyan JIANG
;
Guangping YAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2006;10(43):225-228
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: The relationship of trace element and human body health is increasingly paid more attention. Many trace elements are indispensable for organism. The lack of trace element is closely related to the occurrence of cardio-cerebrovascular disease, while the medicative effect of traditional Chinese medicine depends on the sorts and level of the contained trace elements.OBJECTIVE: To determine the level of Ca, Mg, Cu, Cr, Zn and Fe in 10 traditional Chinese medicine patent prescriptions for the treatment of cerebrovascular disease.SETTING: Medical Experimental Center of Jilin Medical College.MATERIALS: This experiment was carried out in the Jilin Institute of Chemical Technology during March to May 2003. Ten traditional Chinese medicine patent prescriptions Naosaitong, Qiangli Tianma Duzhong Wan,Kangshuan Wan, Tongmai Ningxin Chongji, Rongshuan Jiaonang,Buchang Naoxintong, Dahuoluodan, Zhongfeng Huichun Wan, Xiaoshuan Tongluo Jioonang and Huatuo ZaizaoWan, purchased from market were selected for the treatment of cardio-cerebrovascular disease in this study.Strong HNO3 and grade-guaranteed reagent were made in Beijing Chemical Co. Ltd; HC1O4 and another kind of grade-guaranteed reagent were made in Tianjin Dongfang Chemical Co. Ltd.METHODS: Ten pills and 10 tablets were chosen and crushed evenly respectively. Two portions were equally chosen from each sample, 1.00 geach portion, and placed in ten 50 mL conical flask respectively. Then,strong HNO3 of 10 mL was used to envelop the conical flask, overnight. On the next day, strong HNO3 of 10 mL and HClO4 of 5 mL were respectively added into each flask, then which was slowly heated on the electric hot plate. When about 2 mL liquid was left in the conical flask, another 20 mL strong HNO3 and 5 mL HC1O4 were respectively added to further digest till the solution was achromatic and transparent, and flask was nearly dried.After cooled, the samples were transferred to a 25 mL volumetric flask and metered with 3% HNO3. The level of Ca, Mg, Cu, Cr, Zn and Fe 6 metallic elements in10 traditional Chinese medicine patent prescriptions was measured respectively with atomic absorption spectrophotometry.MAIN OUTCOME MEASURES: The level of Ca, Mg, Cu, Cr, Zn and Fe 6 metallic elements in 10 traditional Chinese medicine patent prescriptions.RESULTS: Among the levels of metallic elements in 10 traditional Chinese medicine patent prescriptions, the level of Mg was found the most in Rongshuan Jiaonang [(89.201 6±0.855 4) mg/g]; The level of Ca the most in Dahuoluodan [(14.312 5±0.507 0) mg/g]; Zn and Fe the most abundant in Kangshuan Wan [(60.487 5±1.639 8)mg/g and(980.081 2±15.395 9) mg/g respectively]; while the level of Cr very more in the Xiaoshuan Tongluo Jiaonang [(9.881 2±0.734 4) mg/g].CONCLUSION: Six metallic elements are all abundant in 10 traditional Chinese medicine patent prescriptions, but significant differences exist.Experimental results provide some evidences for investigating the relationship of the levels of metallic elements in 10 traditional Chinese medicine patent prescriptions and the therapeutic effect on treating cerebrovascular disease.
