Inhibitory effect of triptolide on production of IL -1β from PBMC is associated with IL -1β gene polymorphism
- VernacularTitle:雷公藤甲素抑制PBMC分泌IL-1β蛋白量与 IL-1β基因多态性有关
- Author:
Dongyun SHENG
;
Shenghao TU
;
Hongbo CHEN
;
Yonghong HU
- Publication Type:Journal Article
- Keywords:
Interleukin - 1;
Genes;
Polymorphism(Genetics);
Tripterygium;
Arthritis,rheumatoid
- From:
Chinese Journal of Pathophysiology
2007;23(1):90-94
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore whether the inhibitory effect of triptolide on IL - 1β production by PBMC is asso ciated with IL - 1β gene polymorphisms. METHODS: IL - 1β gene polymorphism was analyzed in 31 healthy volunteers. From genomic DNA, the C - T polymorphism at IL - 1 β - 511 was typed by PCR - RFLP. Meanwhile the IL - 1 β was also measured in the supernatants of the cultured and stimulated peripheral blood mononuclear cells (PBMC) by ELISA. RE SULTS: After LPS stimulation in PBMC cultures of healthy subjects, the secretion levels of IL - 1 β in 9 volunteers who carried IL - 1β -511 T/T genotype were higher than in volunteers who are not T/T genotype (P ＜0.05). Triptolide sup pressed the production of IL - 1β significantly in LPS - treated human PBMC carried C/C and C/T genotype ( P ＜ 0.05 ), but this significant inhibitory effect of triptolide was not seen in T/T genotype ( P ＞ 0.05 ). CONCLUSION: The gene polymorphism at IL - 1β - 511 was related to the production of IL - 1β, and the inhibitory effect of triptolide on the produc tion of IL - 1β was different in C/C, C/T, T/T genotype of IL - 1β -511, which may be one of the reasons for the phe nomenon that people respond differently to triptolide.
