Synergistic protective effect of picroside Ⅱ and NGF on PC12 cells against oxidative stress induced by H2O2
- VernacularTitle:胡黄连甙Ⅱ和神经生长因子对H2O2损伤的PC12细胞的协同保护作用
- Author:
Xiaodong ZHANG
;
Jianwen LIU
;
Ting LI
;
Yan CAO
;
Mingchuan GUO
- Publication Type:Journal Article
- Keywords:
hydrogen peroxide;
nerve growth factor;
picroside II;
reactive oxygen species
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2007;12(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the synergistic protective effect of picrosideII and NGF for the oxidative stress on PC12 cells induced by hydrogen peroxide (H2O2). METHODS: The fluorescent probe 6-carboxy-2',7'-dichlorodihydrofluorescein (CDCFH) was used to assess the intracellular reactiveoxygen species (ROS) level, and MTT assay, morphological observation as well aslactate dehydrogenase (LDH) leakage were conducted to measure cellular injury. RESULTS: The H2O2-induced cytotoxicity was significantly attenuated in the presence of picroside II (25 μg/mL) and NGF (2 ng/mL). Cultures with this combined treatment possessed decreased level of ROS while increased cell survival, as compared to that of picroside II or NGF alone-treated cells. Accordingly, it was concluded that their synergistic protective activities against oxidative stress in vitro were demonstrated in various aspects including reversing morphological changes, enhancingthe ability of cell proliferation and ROS scavenging. CONCLUSION: Such action supports the therapeutic potential of picroside II and NGF in treating nervous disorders based on their synergistic effect.
