Molecular biological research on Borna disease virus infection in depressive patients of Chongqing
- VernacularTitle:重庆地区抑郁症患者博尔纳病病毒感染的分子生物学研究
- Author:
Libo ZHAO
;
Peng XIE
;
Jun MU
;
Yajun LI
;
Qingjun LIU
;
Dezhi ZOU
;
Xiguo ZHOU
;
Jian YANG
- Publication Type:Journal Article
- Keywords:
Depressive Disorder;
Boma disease virus;
fluorescence quantitative polymerase chain reaction;
Chongqing
- From:
Chinese Journal of Nervous and Mental Diseases
2007;33(1):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Background Evidences, from recent studies, suggested that Borna disease virus (BDV) infection might be associated with human neuropsychosis, especially psychiatric disorders including depressive disorder(DD). However, controversy existed about the association between BDV infection and pathogenesis of DD. This study was to explore further whether the infection of Borna disease virus (BDV) is associated with the pathogenesis of depressive disorder (DD).Methods The p 24 fragment of BDV RNA in peripheral blood mononuclear cells (PBMCs) from 60DD patients and 120 healthy volunteers was detected by nested reverse transcriptase polymerase chain reaction (nRT-PCR) combined with fluorescence quantitative polymerase chain reaction (FQPCR). Positive products were cloned and sequenced before being compared with Strain V and strain He/80, from humans and animals.Results The positive rate (5%, 3/60) of BDV p 24 in PBMCs from the DD patients was significantly higher than that (0%, 0/120) from healthy volunteers ( P＜0. 05). The gene sequence for the positive products showed BDV p 24 in PBMCs from DD patients in Chongqing was most homophylic with H1766 strain detected from iii horses (97.68%), with 2 situs mutations (nt 1675 T→C, nt 1678 C→T), and also similar to the standard strain V(96. 51%)and He/80(95.35 %), with basic exchanges limited to T- C and A→G.Conclusions There was BDV infection in the DD patients in China, which indicated that the pathogenesis of DD in human beings in Chongqing might be associated with the infection of BDV.
